A positive feedback loop between TAZ and miR-942-3p modulates proliferation, angiogenesis, epithelial-mesenchymal transition process, glycometabolism and ROS homeostasis in human bladder cancer

Abstract Background Transcriptional coactivator with PDZ-binding motif (TAZ) has been reported to be involved in tumor progression, angiogenesis, epithelial-mesenchymal transition (EMT), glycometabolic modulation and reactive oxygen species (ROS) buildup. Herein, the underlying molecular mechanisms of TAZ-induced biological effects bladder cancer were discovered. Methods qRT-PCR, western blotting immunohistochemistry performed determine levels TAZ cells tissues. CCK-8, colony formation, tube wound healing Transwell assays flow cytometry used evaluate functions TAZ, miR-942-3p growth arrest-specific 1 (GAS1). QRT-PCR expression related genes. Chromatin immunoprecipitation a dual-luciferase reporter assay confirm interaction between miR-942. In vivo tumorigenesis colorimetric glycolytic also conducted. Results We confirmed upregulation vital roles cancer. amplified upstream signaling by inhibiting large suppressor 2 (LATS2, inhibitor). MiR-942-3p attenuated impacts on cell proliferation, EMT, glycolysis ROS induced knockdown. Furthermore, restrained GAS1 modulate behaviors. Conclusion Our study identified novel positive feedback loop that regulates via illustrated might potential therapeutic targets for treatment.